Polymers in Medicine

Polim. Med.
Index Copernicus (ICV 2022) – 121.55
MEiN – 70
Average rejection rate – 39.13%
ISSN 0370-0747 (print)
ISSN 2451-2699 (online) 
Periodicity – biannual

Download original text (EN)

Polymers in Medicine

2020, vol. 50, nr 1, January-June, p. 41–51

doi: 10.17219/pim/128378

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

Preparation and characterization of poly(ethylene oxide)/zinc oxide nanofibrous scaffold for chronic wound healing applications

Hamed Nosrati1,A,B,C,D,E,F, Mohammad Khodaei2,A,B,C,E,F, Mehdi Banitalebi-Dehkordi3,C,E,F, Morteza Alizadeh1,B,C,F, Shiva Asadpour1,3,F, Esmaeel Sharifi4,A,F, Jafar Ai5,A,C,E,F, Mostafa Soleimannejad1,3,A,B,C,D,E,F

1 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran

2 Department of Materials Science and Engineering, Golpayegan University of Technology, Golpayegan, Iran

3 Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

4 Department of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran

5 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background. Skin, the first barrier to pathogens, loses its integrity and function after an injury. The presence of an antibacterial dressing at the wound site may prevent bacterial invasion and also improve the healing process.
Objectives. The current study aimed to fabricate a biomimetic membrane with antibacterial properties for healing chronic wounds.
Material and Methods. The membranes, fabricated through electrospinning, are comprised of poly(ethylene oxide) (PEO) and zinc oxide nanoparticles (ZnO-NPs) as the main biomaterial and antibacterial agent, respectively. Antibacterial activity, cell attachment and viability were tested to evaluate the biological properties of the membranes. The optimal cell compatible concentration of ZnO-NPs was determined for further studies. In vitro characterization of the membranes was performed to confirm their suitable properties for wound healing.
Results. The antibacterial PEO/ZnO-NP membrane containing 2% of nanoparticles showed no cell toxicity, and human fibroblast cells were able to adhere and proliferate on the scaffold. The in vitro results from the tensile test, wettability, porosity, and protein adsorption revealed appropriate properties of the membrane as a scaffold for skin tissue engineering.
Conclusion. Synthetic polymers have been widely used for tissue engineering applications. The proper characteristics of PEO nanofibers, including a high ratio of surface/volume, moderate hydrophilicity and good mechanical properties, make this polymer interesting for skin regeneration. The results demonstrate the potential of the antibacterial PEO/ZnO-NP membrane to be used as an engineered scaffold to improve the wound healing process.

Key words

zinc oxide, wound healing, antibacterial, electrospun membrane, poly(ethylene oxide)

References (50)

  1. Ehterami A, Salehi M, Farzamfar S, et al. Chitosan/alginate hydrogels containing alpha-tocopherol for wound healing in rat model. J Drug Deliv Sci Technol. 2019;51:204–213.
  2. Dash BC, Setia O, Gorecka J, et al. A dense fibrillar collagen scaffold differentially modulates secretory function of iPSC-derived vascular smooth muscle cells to promote wound healing. Cells. 2020;9(4):966.
  3. MacEwan MR, MacEwan S, Kovacs TR, Batts J. What makes the optimal wound healing material? A review of current science and introduction of a synthetic nanofabricated wound care scaffold. Cureus. 2017;9(10):e1736.
  4. Keirouz A, Chung M, Kwon J, Fortunato G, Radacsi N. 2D and 3D electrospinning technologies for the fabrication of nanofibrous scaffolds for skin tissue engineering: A review. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2020;12(4):e1626.
  5. Amiri N, Rozbeh Z, Afrough T, Sajadi Tabassi SA, Moradi A, Movaffagh J. Optimization of chitosan-gelatin nanofibers production: Investigating the effect of solution properties and working parameters on fibers diameter. BioNanoScience. 2018;8(3):778–789.
  6. Vasita R, Katti DS. Nanofibers and their applications in tissue engineering. Int J Nanomedicine. 2006;1(1):15–30.
  7. Okutan N, Terzi P, Altay F. Affecting parameters on electrospinning process and characterization of electrospun gelatin nanofibers. Food Hydrocolloids. 2014;39:19–26.
  8. Miguel S, Ribeiro M, Coutinho P, Correia I. Electrospun polycaprolactone/aloe vera chitosan nanofibrous asymmetric membranes aimed for wound healing applications. Polymers (Basel). 2017;9(5):183.
  9. Miguel SP, Moreira AF, Correia IJ. Chitosan based-asymmetric membranes for wound healing: A review. Int J Biol Macromol. 2019;127:460–475.
  10. Duan B, Dong C, Yuan X, Yao K. Electrospinning of chitosan solutions in acetic acid with poly(ethylene oxide). J Biomater Sci Polym Ed. 2004;15(6):797–811.
  11. Augustine R, Malik HN, Singhal DK, et al. Electrospun polycaprolactone/ZnO nanocomposite membranes as biomaterials with antibacterial and cell adhesion properties. J Polym Res. 2014;21(3):347.
  12. Ahmed R, Tariq M, Ali I, et al. Novel electrospun chitosan/polyvinyl alcohol/zinc oxide nanofibrous mats with antibacterial and antioxidant properties for diabetic wound healing. Int J Biol Macromol. 2018;120(Pt A):385–393.
  13. Hwang J-J, Ma T-W. Preparation, morphology, and antibacterial properties of polyacrylonitrile/montmorillonite/silver nanocomposites. Mater Chem Phys. 2012;136(2–3):613–623.
  14. Sharma M, Yadav S, Ganesh N, Srivastava MM, Srivastava S. Biofabrication and characterization of flavonoid-loaded Ag, Au, Au–Ag bimetallic nanoparticles using seed extract of the plant Madhuca longifolia for the enhancement in wound healing bio-efficacy. Prog Biomater. 2019;8(1):51–63.
  15. Zafari M, Mansouri Boroujeni M, Omidghaemi S, et al. Physical and biological properties of blend-electrospun polycaprolactone/chitosan-based wound dressings loaded with N-decyl-N, N-dimethyl-1-decanaminium chloride: An in vitro and in vivo study. J Biomed Mater Res B Appl Biomater. 2020;108(8):3084–3098.
  16. Antunes BP, Moreira AF, Gaspar V, Correia I. Chitosan/arginine-chitosan polymer blends for assembly of nanofibrous membranes for wound regeneration. Carbohydr Polym. 2015;130:104–112.
  17. Liverani L, Lacina J, Roether JA, et al. Incorporation of bioactive glass nanoparticles in electrospun PCL/chitosan fibers by using benign solvents. Bioact Mater. 2018;3(1):55–63.
  18. Samzadeh-Kermani A, Miri S. Synthesis, characterization and bactericidal property of chitosan-graft-polyaniline/montmorillonite/ZnO nanocomposite. Korean J Chem Eng. 2015;32(6):1137–1141.
  19. Anžlovar A, Orel ZC, Kogej K, Žigon M. Polyol-mediated synthesis of zinc oxide nanorods and nanocomposites with poly(methyl methacrylate). J Nanomater. 2012;2012:31.
  20. Kooti M, Naghdi Sedeh A. Microwave-assisted combustion synthesis of ZnO nanoparticles. J Chem. 2012;2013:562028.
  21. Fradique R, Correia TR, Miguel S, et al. Production of new 3D scaffolds for bone tissue regeneration by rapid prototyping. J Mater Sci Mater Med. 2016;27(4):69.
  22. Lin X, Li Y, Luo W, et al. Leucine-activated nanohybrid biofilm for skin regeneration via improving cell affinity and neovascularization capacity. J Mater Chem B. 2020;8(35):7966–7976.
  23. Chang W, Ma G, Yang D, Su D, Song G, Nie J. Electrospun ultrafine composite fibers from organic‐soluble chitosan and poly(ethylene oxide). J Appl Polym Sci. 2010;117(4):2113–2120.
  24. Cha JH, Didwal PN, Kim JM, Chang DR, Park C-J. Poly(ethylene oxide)-based composite solid polymer electrolyte containing Li7La3Zr2O12 and poly(ethylene glycol) dimethyl ether. J Membrane Sci. 2020;595:117538.
  25. Son WK, Youk JH, Lee TS, Park WH. The effects of solution properties and polyelectrolyte on electrospinning of ultrafine poly(ethylene oxide) fibers. Polymer. 2004;45(9):2959–2966.
  26. Bray R. Antibacterial wound dressing. Google Patents; 2014.
  27. Kazımoğlu H, Uysal E, Dokur M, Günerkan RH. Evaluation of the relationship between neutrophil lymphocyte ratio and the most common bacterial urinary tract infections after transplantation. Bratisl Lek Listy. 2019;120(2):161–165.
  28. Bui VKH, Park D, Lee Y-C. Chitosan combined with ZnO, TiO2 and Ag nanoparticles for antimicrobial wound healing applications: A mini review of the research trends. Polymers (Basel). 2017;9(1):21.
  29. Zhang X, Ren S, He X, et al. Preparation and characterization of cellulose nanofiber/zinc oxide composite films. J Biobased Mater Bioenergy. 2020;14(2):203–208.
  30. Xia T, Kovochich M, Liong M, et al. Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano. 2008;2(10):2121–2134.
  31. Lin W, Xu Y, Huang C-C, et al. Toxicity of nano-and micro-sized ZnO particles in human lung epithelial cells. J Nanoparticle Res. 2009;11(1):25–39.
  32. Bondarenko O, Juganson K, Ivask A, Kasemets K, Mortimer M, Kahru A. Toxicity of Ag, CuO and ZnO nanoparticles to selected environmentally relevant test organisms and mammalian cells in vitro: A critical review. Arch Toxicol. 2013;87(7):1181–1200.
  33. Ghalei S, Nourmohammadi J, Solouk A, Mirzadeh H. Enhanced cellular response elicited by addition of amniotic fluid to alginate hydrogel-electrospun silk fibroin fibers for potential wound dressing application. Colloids Surf B Biointerfaces. 2018;172:82–89.
  34. Dill V, Mörgelin M. Biological dermal templates with native collagen scaffolds provide guiding ridges for invading cells and may promote structured dermal wound healing. Int Wound J. 2020;17(3):618–630.
  35. Maione-Silva L, de Castro EG, Nascimento TL, et al. Ascorbic acid encapsulated into negatively charged liposomes exhibits increased skin permeation, retention and enhances collagen synthesis by fibroblasts. Sci Rep. 2019;9(1):522.
  36. Niu X, Wei Y, Liu Q, et al. Silver-loaded microspheres reinforced chitosan scaffolds for skin tissue engineering. Eur Polym J. 2020;134:109861.
  37. Elsner JJ, Zilberman M. Novel antibiotic-eluting wound dressings: An in vitro study and engineering aspects in the dressing’s design. J Tissue Viability. 2010;19(2):54–66.
  38. Bianco A, Calderone M, Cacciotti I. Electrospun PHBV/PEO co-solution blends: Microstructure, thermal and mechanical properties. Mater Sci Eng C Mater Biol Appl. 2013;33(3):1067–1077.
  39. Dong H, Fey E, Gandelman A, Jones WE. Synthesis and assembly of metal nanoparticles on electrospun poly(4-vinylpyridine) fibers and poly(4-vinylpyridine) composite fibers. Chem Mater. 2006;18(8):2008–2011.
  40. Radwan-Pragłowska J, Janus L, Piatkowski M, Bogdal D, Matýsek D. Hybrid bilayer PLA/chitosan nanofibrous scaffolds doped with ZnO, Fe3O4, and Au nanoparticles with bioactive properties for skin tissue engineering. Polymers (Basel). 2020;12(1):159.
  41. Doulabi AH, Mirzadeh H, Imani M, Bagheri-Khoulenjani S. Chitosan/polyethylene glycol fumarate blend films for wound dressing application: In vitro biocompatibility and biodegradability assays. Prog Biomater. 2018;7(2):143–150.
  42. Ababzadeh S, Farzin A, Goodarzi A, et al. High porous electrospun poly(ε-caprolactone)/gelatin/MgO scaffolds preseeded with endometrial stem cells promote tissue regeneration in full-thickness skin wounds: An in vivo study. J Biomed Mater Res B Appl Biomater. 2020;108(1):2961–2970.
  43. Tserepi A, Gogolides E, Bourkoula A, et al. Plasma nanotextured polymeric surfaces for controlling cell attachment and proliferation: A short review. Plasma Chem Plasma Processing. 2015;36.
  44. Rahimipour S, Salahinejad E, Sharifi E, Nosrati H, Tayebi L. Structure, wettability, corrosion and biocompatibility of nitinol treated by alkaline hydrothermal and hydrophobic functionalization for cardiovascular applications. Appl Surface Sci. 2020;506:144657.
  45. Talebi A, Labbaf S, Karimzadeh F. Polycaprolactone-chitosan-polypyrrole conductive biocomposite nanofibrous scaffold for biomedical applications. Polym Composites. 2020;41(2):645–652.
  46. Kumbar SG, Nukavarapu SP, James R, Nair LS, Laurencin CT. Electrospun poly(lactic acid-co-glycolic acid) scaffolds for skin tissue engineering. Biomaterials. 2008;29(30):4100–4107.
  47. Miguel SP, Ribeiro MP, Brancal H, Coutinho P, Correia IJ. Thermoresponsive chitosan-agarose hydrogel for skin regeneration. Carbohydr Polym. 2014;111:366–373.
  48. Ennaceri H, Wang L, Erfurt D, et al. Water-resistant surfaces using zinc oxide structured nanorod arrays with switchable wetting property. Surf Coat Technol. 2016;299:169–176.
  49. Jones V, Grey JE, Harding KG. ABC of wound healing: Wound dressings. BMJ. 2006;332(7544):777–780.
  50. Arima Y, Iwata H. Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers. Biomaterials. 2007;28(20):3074–3082.
© Wroclaw Medical University