Polymers in Medicine

Polim. Med.
Scopus CiteScore: 3.5 (CiteScore Tracker 3.6)
Index Copernicus (ICV 2023) – 121.14
MEiN – 70
ISSN 0370-0747 (print)
ISSN 2451-2699 (online) 
Periodicity – biannual

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Polymers in Medicine

2009, vol. 39, nr 4, October-December, p. 61–67

Publication type: original article

Language: Polish

Elektroforeza w octanie celulozy CAE w badaniach komercyjnych alginianowych opatrunków aktywnych

Electrophoresis on a cellulose acetate CAE in a study of commercial active alginate dressings

Anna Pielesz1,

1 Wydział Nauk o Materiałach i Środowisku Instytut Inżynierii Tekstyliów i Materiałów Polimerowych, Akademia Techniczno-Humanistyczna w Bielsku-Białej

Streszczenie

Hydrożele zbudowane są z trójwymiarowej sieci polimerów polisacharydowych, które magazynują znaczną ilość wody, zapewniają nawilżenie i nawodnienie suchej rany. Jedną z najważniejszych właściwości hydrożeli alginianowych jest zdolność do absorpcji, uwalniania i retencji związków chemicznych, w tym głównie wody. W pracy dokonano identyfikacji metodą elektroforezy w octanie celulozy CAE rozpuszczalnych w wodzie alginianów sodu, zawartych w opatrunkach aktywnych Medisorb A. Wykazano również obecność kwasów guluronowego (G) i manuronowego (M) w hydrolizatach wzorca kwasu alginowego AA i alginianowych opatrunkach aktywnych Medisorb A. Zaobserwowano metodą elektroforezy CAE i dokonano próby interpretacji proponowanych wcześniej w literaturze tzw. struktur „egg-box” („jajko w pudełku na jajka” lub „wytłoczka do jajek”). Stwierdzono, że metoda elektroforezy CAE powinna znaleźć szersze zastosowanie do badań biomateriałów na bazie alginianów.

Abstract

Hydrogels are cross-linked threedimensional macromolecular networks that contain a large fraction of water within their structure. One of the most important properties of alginate hydrogels, leading to their broad versatility, is their ability for controlled uptake, release and retention of molecules. This ability, in turn, is due to specific interactions of the macromolecular network with the diffusing or retained molecule, for example ions. In this study, water-soluble sodium alginates contained in active dressings Medisorb A were identified using electrophoresis on cellulose acetate CAE. The presence of guluronic acid (G) and mannuronic acid (M) residues in hydrolysates of alginic acid AA (used as reference substance) and active alginate dressings Medisorb A was also proved. “Eggbox” structures, earlier described in literature, were observed using the same technique and their interpretation was attempted. Electrophoresis on cellulose acetate was found to be a useful method for examining alginatebased biomaterials.

Słowa kluczowe

elektroforeza w octanie celulozy CAE, alginianowe opatrunki aktywne, kwas guluronowy (G) i kwas manuronowy (M)

Key words

electrophoresis on a cellulose acetate CAE, active alginate dressings, guluronic acid (G) and mannuronic acid (M)

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